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example

Example

Let's start by assessing relatedness between your favorite genome (MyGenome) and genomes available in public databases. Example directory contains 14 fasta files of genomes in zip archive 14gx.zip :

cd ki-s/example

Calculation of the percentage of shared 15-mers between all genomes :

../ki-s.pl -z gx14.zip -m kmers -k 15 -o gx14k15

Generate circle packing representation of this matrix (informations to color points are in gx14_infos.tab file) :

../tools/generate_packing.pl -in gx14k15_matrix_kmers.csv -car gx14_infos.tab -o=gx14k15_circ -thrs='0.5;0.8;0.9'

Open gx14k15_circ/gx14k15_circ.html with a web browser, you can see your favorite genome (MyGenome) is a Xylella fastidiosa subsp. pauca strain.

You can also compute ANIb (even if it is more time-consuming) :

../ki-s.pl -z gx14.zip -m ani -bl=ANIb -o gx14anib

And create circle packing representation of this matrix :

../tools/generate_packing.pl -in gx14anib_matrix_ani.csv -car gx14_infos.tab -o=gx14anib_circ -thrs='0.95;0.98;0.99'
  

Open gx14anib_circ/ gx14anib_circ.html with a web browser, ANIb confirm that MyGenome is a Xylella fastidiosa subsp. pauca strain.

example.txt · Dernière modification : de martial.briand@inrae.fr